Changes in the root fungal microbiome of strawberry following application of residues of the biofumigant oilseed radish

Biofumigation has been proposed as an environmentally friendly method of plant protection against soil-borne pathogens, but its effects on microbial communities are still incompletely understood. Using high throughput DNA sequencing, we investigated the oilseed radish residues root fungal microbiome strawberry in presence a pathogen, Verticillium dahliae. Results our greenhouse study show that early flowering occurred response to residue addition, suggesting stress-response and there was significant decrease berry yield. The roots significantly restructured by both biofumigation inoculation with Verticillium. In particular, abundance endophyte- arbuscular mycorrhizal functional guilds reduced result biofumigant V. dahliae whereas saprotrophs increased when treatments were applied together. Alpha diversity analyses fungi associated indicated increase species richness following inoculation, alone or resulted no effect, apparently some rare taxa may have enriched/stimulated pathogen. Further investigations should reveal whether negative potentially beneficial endophytes host genotype- soil-dependent.